Functional characterization of Arabidopsis thaliana GAT1 in heterologous expression systems, i. In contrast to the most closely related proteins of the proline transporter family, proline and glycine betaine are not transported by AtGAT1. AtGAT1 does not share sequence similarity with any of the non-plant GABA transporters described so far, and analyses of substrate selectivity and kinetic properties showed that AtGAT1-mediated transport is similar but distinct from that of mammalian, bacterial, and S. Although GABA was discovered in as a constituent of potato tubers, research on GABA metabolism and transport advanced much faster in the animal system as GABA turned out to be the most abundant inhibitory neurotransmitter in the central nervous system 1. In addition to its function as a neurotransmitter, GABA plays a role in the development of the nervous system, influencing proliferation, migration, and differentiation 4. In both bacteria and yeast, GABA uptake and biosynthesis are mainly involved in nitrogen and carbon metabolism 7 - 9 , although other functions such as GABA synthesis for pH regulation in Escherichia coli and for normal oxidative stress tolerance in S. Much less is known about the role of GABA and its transport across the plasma membrane in plants. GABA rapidly accumulates under various stress conditions such as low temperature, mechanical stimulation, and oxygen deficiency 12 ,
Browsing Doctoral Dissertations by Author "Cortes Bermudez, Diego Fernando"
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Energy from the Sun pp Cite as. Although the four isoforms share a high degree of identity, the recombinant NADP-ME1 to 4 show well-distinct kinetic and structural properties. When analyzing the activity of each isoform in the presence of possible metabolic effectors, the results obtained indicate that NADP-ME2 is the most highly regulated isoform, especially by activation. The four isoforms behave differently in terms of reversibility, presenting NADP-ME4 the highest ratio between the reverse — carboxylation and reduction of pyruvate — to the forward reaction. In order to identify residues or segments of the primary structure of each NADPME isoform that could be involved in the differences in kinetic and regulatory properties among the isoforms, NADP-ME2 mutants and deletions were constructed and analysed. The results obtained show that Arg is involved in fumarate activation, while the regions involved in aspartate and CoA modulation are located at the amino-terminal part of the protein. On overall, these studies show that minimal structural changes are responsible for the different kinetic behaviour of each AtNADP-ME isoform. Unable to display preview. Download preview PDF.
EXPERIMENTAL PROCEDURES
The study of individual plant cells and their growth structure is an important focus of research in plant genetics. To obtain development information at cellular level, researchers need to perform in vivo imaging of the specimen under study, through time-lapse confocal microscopy. Within this research field it is important to understand mechanisms like cell division and elongation of developing cells. We describe a tool to automatically search for cell division in the Arabidopsis thaliana using information of nuclei shape. The nuclei detection is based on a convergence index filter.
I felt insane and crazy for being upset and considered just leaving ASAP. It's pretty rough waking up to drive home that early and then trying to go back to sleep again for just a little bit before getting up again for work. And even longer when you add that one-year fellowship to the end of it.
